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Gel Electrophoresis
Agarose gel electrophoresis, method to separate mixed popular of DNA
 PAGE, technique to separate proteins and nucleic acid, according to electrophoretic mobility |  Agarose gel electrophoresis, methods to separate mixed population of DNA |  Application in Genetic Diagnosis and DNA Fingerprinting |  Fluorescent Chemical is Used For visualization of Molecules |
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Gel Electrophoresis
Polyacrylamide gel electrophoresis
Pros
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The pore size of acrylamide are uniform.
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It is inert and not react with sample since there is no charge associated with it.
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Very high resolving power.
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Can increase or decrease molecular sieving by manipulate the pore sizes.
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Different buffers can be used to manipulate resolution and run time.
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Chemically synthethic gel can give more reproducible results.
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Promote better separations at higher field strengths due to efficient heat dissipation of thin gels.
Cons
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Acrylamide and TEMED are neurotoxic compounds. Gloves should be wear when handling solutions of acrylamide and TEMED.
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Longer wait time for gel to set.
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More care needed when pouring the gel to ensure gas bubbles are removed.
Structure of TEMED (neurotoxic)
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