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Polyacrylamide gel electrophoresis

Pros

  1. The pore size of acrylamide are uniform.

  2. It is inert and not react with sample since there is no charge associated with it.

  3. Very high resolving power.

  4. Can increase or decrease molecular sieving by manipulate the pore sizes.

  5. Different buffers can be used to manipulate resolution and run time.

  6. Chemically synthethic gel can give more reproducible results.

  7. Promote better separations at higher field strengths due to efficient heat dissipation of thin gels.

 

 

 

Cons

  1. Acrylamide and TEMED are neurotoxic compounds. Gloves should be wear when handling solutions of acrylamide and TEMED.

  2. Longer wait time for gel to set.

  3. More care needed when pouring the gel to ensure gas bubbles are removed.

Structure of TEMED (neurotoxic)

 

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